Isolation and characterization of lamellar body material from rat lung homogenates by continuous linear sucrose gradients.
A technique is described for isolating lamellar body material from rat lung.Membranes with relative densities Order Racks ranging between 1.050 and 1.074 g/ml were isolated by centrifugation of crude lung homogenates upward through continuous linear sucrose gradients at 40,000 rpm (199,000 g) for 3 hr.Their protein and lipid content was characteris